Scientist III, Protein Biology

Overview

Scientist III, Protein Biology on the Antibody team, engineering and developing antibodies with a focus on reagent quality and specificity, using display-based screening and validation methods.

What you'll do

• Develop and implement experimental strategies for phage display projects, including library construction, selection, and screening.
• Design and optimize assays for protein binding or activity measurements.
• Perform phage display selections to isolate specific binding or functional interactions.
• Develop and execute screening protocols, including affinity-based selections or functional assays.
• Perform molecular biology techniques such as PCR, DNA cloning, protein expression, and purification, and implement quality control to validate constructs and protein production.
• Use phage display to engineer proteins with improved properties, such as binding affinity, specificity, or stability, applying directed evolution or rational design.
• Analyze and interpret experimental data using statistical methods and bioinformatics tools to identify binding or functional motifs and propose protein engineering strategies.
• Collaborate with multidisciplinary teams, including scientists from biology, chemistry, and bioinformatics, and communicate findings through oral presentations and written reports.
• Maintain and operate required laboratory equipment, ensure safety compliance, maintain accurate records, and manage inventory and ordering of reagents and supplies.
• Review scientific literature and apply new methodologies to improve experimental workflows.
• Identify and troubleshoot technical issues in phage display experiments and develop solutions to optimize protocols.

What you'll need

• Master's degree with greater than 5 years of relevant experience, or PhD with greater than 2 years of experience, in a life sciences field with a strong focus on structural biology, protein design, protein purification, molecular biology, immunology, biotechnology or biochemistry, and experience with antibody development technologies.
• Proficiency in display-based antibody development techniques, with a strong track record of successful projects.
• In-depth knowledge of display platforms such as phage display, yeast display, or bacterial display.
• Experience in antibody library construction, screening, and optimization using display-based methods.
• Familiarity with antibody engineering and optimization strategies, including affinity maturation and humanization.
• Proficiency in molecular biology techniques, including DNA manipulation, cloning, and protein expression.
• Strong analytical and problem-solving skills, with the ability to troubleshoot and optimize display-based antibody development protocols.
• Excellent communication and collaboration skills for cross-functional teams.
• Attention to detail and ability to meticulously analyze and interpret experimental data.
• Flexibility and adaptability to work in a fast-paced research environment.
• Proficiency in MS Office applications such as Word, PowerPoint and Excel, with strong statistical knowledge and familiarity with statistical software such as Prism.
• Self-motivated, with excellent oral and written communication skills.

Details

• Location: Bangalore, India.
• Standard work schedule, Monday to Friday.
• Work involves a laboratory setting with adherence to Good Manufacturing Practices (GMP) and safety standards, including use of personal protective equipment, and working with hazardous or toxic materials.
• Environmental conditions include cold rooms and freezers (-6 degrees C / -22 degrees F), fluctuating temperatures, loud equipment noise, rapid flashing lights, and strong chemical, lubricant and biological odors.